These protocols are routinely used in the lab. They are from many different sources. Where known, references are included with the protocol.

Frequent sources of protocols:

Experiments with Gene Fusions. Silhavy, T., Berman, M., and Enquist, L. Cold Spring Harbor Laboratory. 1984. Cold Spring Harbor, NY.

A Short Course in Bacterial Genetics. Miller, J. Cold Spring Harbor Laboratory. 1992. Cold Spring Harbor, NY.

Molecular Cloning. Sambrook, J., Fritsch, E., Maniatis, T. Cold Spring Harbor Laboratory. 1989. Cold Spring Harbor, NY. (there are more current editions of this, too)

Advanced Bacterial Genetics. Davis, R., Botstein, D., and Roth, J. Cold Spring Harbor Laboratory. 1980. Cold Spring Harbor, NY.

Protocols

Affinity Column - Preparation of Resin

Annealing Oligos

b-galactosidase Assays

Camphor Assay-liquid and plate

Chloroquine Gels

Coomassie Staining of Protein Gels

DAPI Staining of Cells

Disk Assay for Sensitivity to Toxic Compounds

DNA Labeling with HhaI -3H-SAM

Electroporation

Ethidium Bromide Restriction Digests (Random Partials)

Filter Binding Assay

Flow Cytometry - Growing Cells for

Frozen Glycerol Stocks of Bacteria

Fusions to lacZ using Hybrid Transposons

Growth Rate Media

Hop Pools

Immunoprecipitations Using Protein G Sepharose

Induction of Arabinose Inducible Fusions for b-galactosidase Assays

Kinase Reactions

Measuring DNA Synthesis

Northern Blots

Nucleoids (High Salt and Spermidine Preps)

P1 Lysates

P1 Transductions

PCR General Procedure

Paint Pellet DNA Precipitations

Phage Lysates from Plaques

Phage Plate Lysates

Phenol Extraction and Ethanol Precipitation of DNA

Plasmid Copy Number Determinations

Protein Extracts (French Press, PEI precipitation, TCA Precipitation)

Southern Blots + ECL Detection

Topoisomerase Assays

TSS Quick Transfomation of DNA

UV Induction of Lambda

Western Blots

Western Blots - NuPAGE Gels

X-Ray Irradiation Protocol